Frank Collart

Biosciences Division Bldg: 202 Room: A-357 Email: fcollart@anl.gov Phone: (630) 252-4859 Fax: (630) 252-5517 Education Professional Experience Publication List Research Highlights

> Education:

• 1984, Ph.D, Medical College of Ohio, Medical Sciences
• 1982, M.S., Bowling Green State University, Chemistry
• 1977, B.A., Bowling Green State University, Chemistry

> Professional Experience:

1994-present:	Molecular Biologist; Biosciences Division, Argonne National Laboratory.
1989-1994:	Assistant Molecular Biologist; Division of Biological and Medical Research, Argonne National Laboratory.
1984-1989:	Postdoctoral Appointee, Supervisor: Dr Eliezer Huberman; Division of Biological and Medical Research, Argonne National Laboratory.

> Memberships in professional societies:

• Sigma Xi
• American Association for Cancer Research
• American Society for Microbiology

> Review committees:

• Medical Advisory Board of the Leukemia Research Foundation, 1996-Present
• American Cancer Society, Illinois Division Research Advisory Committee, 1998-Present
• Molecular Biology and Genetics-2 (MBG-2).  Peer review panel of the 2008 Prostate Cancer Research Program (PCRP), of the Congressionally Directed Medical Research Programs (CDMRP), Department of Defense (DoD)
• Advanced Photon Source sector review panel: 2004-2006.
• Manuscript reviewer for Biochemistry, PNAS, Protein Science, Nucleic Acids Research, Applied and Environmental Microbiology, Biotechniques, Journal of Integrative Biology, Journal of Biological Chemistry.

> Publications (Last 3 years):

1. Yuri Y. Londer, Sarah E. Giuliani, Terese Peppler, Frank R. Collart. Addressing Shewanella oneidensis “cytochromome”: the first step towards high-throughput expression of cytochromes c. Protein Expression and Purification,  E-published Jul 8, 2008
2. Younggyu Kim, Sam On Ho, Natalie R. Gassman, You Korlann, Elizabeth V. Landorf, Frank Collart, and Shimon Weiss.  Efficient site-specific labeling of proteins via cysteines. Bioconjugate Chemistry 3 786-91, 2008.
3. Structural Genomics Consortium; China Structural Genomics Consortium; Northeast Structural Genomics Consortium, Gräslund S, Nordlund P, Weigelt J, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC.  Protein production and purification.  . Nat Methods. 5(2):135-46, 2008.
4. Vorontsov II, Minasov G, Brunzelle JS, Shuvalova L, Kiryukhina O, Collart FR, Anderson WF. Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity. Protein Sci. 2007, 11:2483-2490.
5. Nocek B, Mulligan, R., Bargassa, M., Collart, F., and Joachimiak A.  Crystal Structure of Aminopeptidase N from Human Pathogen Neisseria meningitides. Proteins. 2008, 70:273-9.
6. Kolker, E., Hogan, J.M., Higdon, R., Kolker, N., Landorf, E., Yakunin, A.F., Collart, F.R., van Belle, G.  Development of BIATECH-54 standard mixtures for assessment of protein identification and relative expression.  Proteomics. 2007, 20:3693-8.
7. Michael H. Godsey, George Minasov, Ludmilla Shuvalova, Joseph S. Brunzelle, Ivan I. Vorontsov, Frank R. Collart, Wayne F. Anderson. The 2.2 Å Resolution Crystal Structure of Bacillus cereus Nif3-family protein YqfO, reveals a conserved dimetal-binding motif and a regulatory domain.  Protein Sci. 2007 (7):1285-93.
8. Stols, L.;Zhou, M.; Eschenfeldt, W.H.; Sanville Millard, C.; Abdullah, J.; Collart, F.R.; Kim, Y., Donnelly, M.I.  New vectors for co-expression of proteins: Structure of Bacillus subtilis ScoAB obtained by high throughput protocols.  Protein Expr Purif. 2007 53(2):396-403.
9. Gerdts CJ, Tereshko V, Yadav MK, Dementieva I, Collart F, Joachimiak A, Stevens RC, Kuhn P, Kossiakoff A, Ismagilov RF.   Time-Controlled Microfluidic Seeding in nL-Volume Droplets to Separate Nucleation and Growth Stages of Protein Crystallization. Angew Chem Int Ed Engl. 2006 45(48):8156-60.
10. Osipiuk J, Maltseva N, Dementieva I, Clancy S, Collart F, and Joachimiak A.  Structure of YidB protein from Shigella flexneri shows a new fold with homeodomain motif. Proteins. Proteins. 65(2):509-13, 2006.
11. Rajan, S.S, Yang, X., Shuvalova, L., Collart, F., Anderson, W.F.  Crystal structure of YfiR, an unusual TetR/CamR-type putative transcriptional regulator from Bacillus subtilis.  Proteins. 2006 65(1):255-7.
12. Dieckman, L.J., Zhang, W., Rodi, D.J., Donnelly, M.I., and Collart, F.R.  Bacterial Expression Strategies for Human Angiogenesis Proteins.  J Struct Funct Genomics. 2006 7(1):23-30.
13. Borowicz, S., Fackenthal, J., Collart, F.R.; Myatt, E., Moy, S., Babnigg, G., Wilton, R., Boernke, W.E., Schiffer, M., Stevens, F.J., Olopade, O.I. Primary structure-based function characterization of BRCT domain replicates in BRCA1.  Biochem Biophys Res Commun. 2006 345(1):188-96.
14. Zhang, R., Joachimiak, G., Jiang, S., Cipriani, A., Collart, F., and Joachimiak, A.  Structure of Phage Protein BC1872 from Bacillus cereus, a singleton with new fold.  Proteins 2006 64(1):280-3.
15. Kim, Y., Maltseva, N., Dementieva, I., Collart, F., Holzle, D., Joachimiak A.  Crystal structure of hypothetical protein YfiH from Shigella flexneri at 2 A resolution.  Proteins 2006 1;63(4):1097-101.
16. Donnelly, M.I., Zhou, M., Sanville Millard, C., Clancy, S., Stols, L., Eschenfeldt, W.H., Collart, F., Joachimiak, A.  An expression vector tailored for large-scale high throughput purification of recombinant proteins. 2006 Protein Expr Purif. 47(2):446-54.
17. Lin, C-T., Moore, P.A., Auberry, D.L., Landorf, E.V, Peppler, T., Victry, K.D., Collart, F.R., and Kery, V.  Automated Purification of Recombinant Proteins: Combining High-Throughput with High Yield. 2006 Protein Expr Purif.  47 (1) 16-24.
18. Dieckman, L.J., Hanly, W.C., and Collart, F.R.  Strategies for High Throughput Gene Cloning and Expression.  Genetic Engineering: Principles and Methods. 2006 Vol. 42 (5) 590-596.
19. Qiu Y, Tereshko V, Kim Y, Zhang R, Collart F, Yousef M, Kossiakoff A, Joachimiak A.  The crystal structure of Aq_328 from the hyperthermophilic bacteria Aquifex aeolicus shows an ancestral histone fold. Proteins. 2006 62(1):8-16.