The Environmental Sample Preparation and Sequencing Facility (ESPSF) at Argonne National Laboratory is a state-of-the-art facility for nucleic acid extraction, library preparation, and ultra-high throughput sequencing. The facility was originally established in 2007 at Argonne National Laboratory and is housed in Argonne’€™s Computing, Environment and Life Sciences (CELS) directorate, providing world-class computation power, software, and expertise critical to the acquisition, analysis, and interpretation of the unprecedented volume of genetic information being generated on next generation DNA sequencing platforms. The facility provides resources and services to Argonne  and University of Chicago users, and to the broader scientific community (both US and abroad). ESPSF is involved in a wide range of scientific research from the sequencing of metagenomic samples to both 16S and 18S rRNA amplicon sequencing for microbial community analysis, and ITS sequencing for fungal community analysis. The ESPSF team helps users determine the most efficient and cost effective approach to meet their research needs.

The ESPSF currently supports the Illumina HiSeq2000 and Illumina MiSeq for high-throughput sequencing. The Illumina HiSeq2000 generates over 6 billion reads at 100 bp in length per read resulting in over 600 Gb worth of data across sixteen lanes available per run. The Illumina MiSeq generates over 24 million reads at 150 bp, 250bp or 300bp in length per read, resulting in over 3.6 Gb, 6.0 Gb or 7.2Gb worth of data available per run (over a single lane). Applications on both the Illumina HiSeq2000 and MiSeq include shotgun metagenome sequencing, metatranscriptome sequencing, rRNA-based amplicon library sequencing, and genome sequencing and resequencing.

Contact:  Sarah Owens,

Shipping Address:
Argonne National Laboratory
9700 S Cass Avenue
Bldg. 446, Rm. A176
Lemont, IL 60439

Shotgun sequencing for microbial community metagenomes
Recommended platform: Illumina HiSeq2000. We recommend no more than 4-8 samples per lane.
Required material: 1ug of DNA

Amplicon library sequencing
Recommended platform: Illumina MiSeq. Results are best when 200-700 samples are sequenced simultaneously in a single pool.

Amplicon library preparation
Required material: 1-50ng/ul of DNA, 20ul volume in 96-well skirted PCR plate

Genome sequencing
Recommended platform: Illumina HiSeq2000 or Illumina MiSeq
Required material: 1ug of DNA

Metatranscriptomic Sequencing
Recommended platform: Illumina HiSeq2000
Required material: 5ug of total RNA