The Structural Biology Center (SBC)  is a national user facility for macromolecular crystallography at the Advanced Photon Source. The facility uses advanced instrumentation , state-of-the-art software, and methods and high throughput technologies. SBC addresses the most challenging projects in structural biology that include large macromolecular assemblies (ribosomes, viruses, regulatory protein/DNA complexes) and membrane-bound and membrane-associated proteins (ion channels, receptors, integrins). Structures of enzymes and complexes with ligands and inhibitors can be determined at atomic resolution.

 

 

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Kim Y, Ye Z, Joachimiak G, Videau P, Young J, Hurd K, Callahan SM, Gornicki P, Zhao J, Haselkorn R, Joachimiak A. (2013) Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets, PNAS USA, 110(19):E1716-23

The SBC enables the atomic-scale study of macromolecular systems using very small crystal samples. It also offers the most efficient data collection and structure determination systems for protein crystallography worldwide. Thanks to recent advances with larger, faster X-ray detectors and automation of laboratory processes for expressing proteins and growing crystals, the time required to solve molecular structures has been greatly reduced. Research that not long ago took months or years may now take only hours. In addition, one need not be a macromolecular crystallographer to take advantage of these facilities; our experienced beamline staff are available to guide even novice users through the entire process.

APCF laboratory

Laboratory at the SBC.

 

More information about the SBC: www.sbc.anl.gov

Interconnections: 

Argonne collaborators:

Frank Collart – Molecular sensors

Christopher Henry – Microbial metabolism, enzyme promiscuity

Karolina Michalska – Marine sediments archaeal proteases

Youngchang Kim – Hypotheticals from select pathogens

Gyorgy Babnigg – Microfluidic devices in functional and structural biology

Outside collaborators:

Robert Haselkorn – Transcription regulation of heterocyst differentiation

Howard Shuman – Legionella secreted effectors

Olaf Schneewind – Virulence factors, Yersinia pestis hypotheticals

Steve Kent – Racemic crystallography

Sean Crosson – Brucella hypotheticals

Michelle O’Malley – Cellulases from anaerobic gut fungi

Matthias Hess – Unusual cellulases

Kirsten Hofmockel – Carbon cycling core enzymes in soil aggregates

 

 

 

Contact: Andrzej Joachimiak, andrzejj@anl.gov