High Throughput Molecular Biology - DOE GENOMICS : GTL

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High Throughput Expression Technology > read more

The development of microwell plate-based methods for the generation of bacterial expression clones enables a genome scale approach to protein synthesis. The availability of these methods in combination with technological advances in universal cloning techniques, expression systems, and bacterial expression strains provides an opportunity to evaluate the effectiveness of present high throughput methods for production of a majority of the proteins encoded in a genome. This genome scale approach will define the how much of the proteome can be expressed as useful expression clones and provide a basis for the evaluation of the cost and coverage of high throughput protein production strategies.

Protein Purification > read more

There is an enormous demand for purified proteins from many experimentalists working in areas of interest to the DOE with current resources and capabilities unable to meet this demand. In part this is a consequence of the rapid development of expression systems, the higher density of cloning experiments relative to purification methods, and the requirement that considerably more effort is expended to produce a purified protein relative to that required for generation of an expression clone.

Resources > read more

An essential component of the project is the transfer of data and physical resources to experimental labs. This process provides validation for the high throughput methods resources generated by the program and provide valuable feedback for project design, workflow management, and resource distribution. These resources include plasmid plates containing various expression constructs, vector and host resources required for experimental work, and the associated project documentation.