Production and functional characterization of proteins
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Frank Collart Bldg: 202. Room: D129 E-mail: fcollart@anl.gov Phone: (630) 252-4859
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> Research Highlights
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Mapping Ligands to Binding Proteins and Domains. Functional assignments of sensor type proteins will be improved by the development of high throughput methods to match ligands with their binding proteins. This project will provide specific functional assignments for an important class of regulatory molecules and generate an expression-clone resource for future structural and functional studies. (Collaborative project with Brookhaven National Lab) | |
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Assignment of Enzymatic Function for Core Metabolic Enzymes. HTP protein production methods and screening systems will be used to identify enzymatic activity for proteins with unknown function. The outcome will provide a foundation to assess the capabilities for specific functional assignments for a substantial number of unknown bacterial and eukaryotic proteins. (Collaborative project with University of Toronto and University of Alabama, Huntsville) | |
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HTP Strategy for Annotation of Fungal Glycosyl Hydrolases. The objective of this project is to use high throughput proteomics, protein expression and enzyme assays to rapidly generate meaningful functional annotation of secreted proteins, specifically fungal glycosyl hydrolases. A variety of substrates and assay methods will be developed for high-throughput functional classification and characterization of fungal secreted enzymes. The focus will be on known and potentially novel glycosyl hydrolase families. (Collaborative project with Pacific Northwest National Lab, Scott Baker, PI). | |
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Zebrafish Protein & Antibody Core. We are evaluating strategies for in vitro cloning and production of proteins from Danio rerio. This project is a component of the NIH funded “Zebrafish Protein and Antibody Core” (Brian Kay, PI) which will generate affinity reagents to zebrafish proteins for use by the research community. There are three goals for this project component: Read more. |
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Development of Genome Scale Expression Methods. This program has focused on the development of high throughput protein expression methods. A central goal is the distribution of clones and proteins to DOE science programs to insure the appropriate technologies are brought to bear on the scientific problem. The current E. coli expression clone library contains over 1500 clones that have been screened for expression and solubility with more than 800 clones that have been validated for successful expression of a soluble protein. Over 500 validate expression clones and ~300 purified proteins have been provided to end users. | |
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Next generation Sequencing Technology Applied to Genome Technology.This project is developing tools to improve genome annotation using next generation sequencing technology combined with unique ANL experimental resources and capabilities. Deep RNA sequencing will be used to generate consensus gene models for evaluation and improvement of gene models currently generated by automated processes. | |
