 Carol Giometti
> Education:
- Knox College B.A. 1972 Biology
- Rush Hospital MT 1973 Medical Technology *
- University of Illinois Ph.D. 1978 Biochemistry
*Certified Medical Technologist; Internship at Rush-Presbyterian St. Luke's Hospital,Chicago, 1972/73
> Professional Experience:
- 2001-present Senior Biochemist and Group Leader, Protein Mapping Group, Argonne National Laboratory
- 1987-2000 Biochemist and Group Leader, Protein Mapping Group, Argonne National Laboratory
- 3/1999-5/2000 Acting Division Director, Biosciences Division, Argonne National Laboratory
- 1985-1987 Assistant Biochemist, Protein Mapping Group, Argonne National Laboratory
- 1982-1985 Assistant Biochemist, Molecular Anatomy Program, Argonne National Laboratory
- 1981-1982 Assistant Biologist, Molecular Anatomy Program, Argonne National Laboratory
- 1978-1981 Postdoctoral Appointee, Molecular Anatomy Program, Argonne National Laboratory
- 1973-1978 Teaching Assistant,University of Illinois at the Medical Center, Chicago
- 1972-1973 Medical Laboratory Intern, Rush-Presbyterian St. Luke's Hospital, Chicago
> Research Highlights:
My current research interests include (1) the detection and characterization of changes in protein expression within biological systems in response to environmental stimuli and disease processes, (2) the development of new approaches to the separation and characterization of proteins in complex mixtures that are compatible with high-throughput research applications, (3) the construction of databases for protein expression data, and (4) the interfacing of the protein expression databases with gene expression and gene sequence databases. Two-dimensional gel electrophoresis (2DE) and HPLC/MS-MS are used as tools for protein separations and identification of specific proteins. Development of methods for separation of native proteins (classical 2DE is designed for the separation of denatured proteins) that will allow the detection and characterization of protein-protein interactions as well as protein function are in progress. Data from this research is being stored using a relational database interfaced with World Wide Web capability for interrogation of the protein expression and identification data in the context of genome sequence information.
See:
http://proteomeweb.anl.gov
http://gelbank.anl.gov
> Patents
Zhang; Jian-Shi, Giometti; Carol S., Tollaksen; Sandra L. 1987. Electrophoretic extraction of proteins from two-dimensional electrophoresis gel spots. No. 4,824,547
> Lab Personnel
View photos
Staff:
- Gyorgy Babnigg, PhD
- Tripti Khare, PhD
- Carl Lindberg, BS
- Sandy Tollaksen, MS
Temporary:
- Angela Ahrendt, PhD
- Shisheng Li, PhD
- Jessica Smotrys, PhD
> Honors/Awards
- Young Investigator Award in Clinical Chemistry, 1984
- American Association of University Women Scholarship, 1977-1978
> Grant Support
U.S. Department of Energy
Analysis of S. oneidensis membrane protein expression in response to electron acceptor availability
The goal of the project is to characterize the environmental regulation of the expression of Shewanella putrefaciens membrane proteins involved in the reduction of metals relevant to bioremediation.
U.S. Department of Energy
The Microbial Proteomics Project: A Database of Microbial Protein Expression in the Context of Genome Analysis
The goal of the project is to explore whole genome sequence information for defining the functions of unknown genes and regulatory networks in dissimilatory metal reduction pathways.
U.S. Department of Energy
Analysis of the Genetic Potential and Gene Expression of Microbial Communities Involved in the in situ Bioremediation of Uranium and Harvesting Electrical Energy from Organic Matter (D. Lovley, U Mass GTL project leader)
The goal of this project, a subtask within the University of Massachusetts Genomes to Life Project, is to identify the proteins up-regulated in different strains of Geobacter sp., both laboratory isolated and environmental samples, in response to specific environmental factors.
U.S. Department of Energy
Genomes to Life Center for Molecular and Cellular Systems: A Research Program for Identification and Characterization of Protein Complexes
The goal of this project, a subtask within the Oak Ridge National Laboratory GTL project, is to identify the protein components in isolated protein complexes.
National Institutes of Health
The Midwest Center for Structural Genomics
The goal of this project, a subtask within the Midwest Center for Structural Genomics, is to identify and characterize protein complexes and their component parts for protein expression.
Argonne Laboratory Directed Research and Development
High-throughput Analysis of Low Abundance Protein Constituents in Complex Biological Mixtures
The goal of this project is to develop protein separation and analysis methods for the high-throughput analysis of proteins expressed in low abundance by a variety of biological systems.
Argonne Laboratory Directed Research and Development
Proteomic and p hage-display analysis of mitochondrial compartmental oxidant biosensors for the study and reversal of sudden death events .
The goal of this project is to adopt proteomic and phage-display approaches to analyze the molecular changes in cardiac mitochondrial proteins during sudden death and resuscitation.
National Institutes of Health
The goal of this research is to develop methods for the analysis of the surface components of neural stem cells. C. Giometti will supervise the separation and identification of proteins expressed on the outer surface of the cultured cells used for this project. |
