David Glesne

Email: glesne@anl.gov STA   ~ Education Professional Experience Publication List Research Highlights Grant Support Lab Personnel

> Education:

• University of Virginia, Charlottesville, VA, B.S. Biology, 1987
• University of Chicago, Chicago, IL, Ph.D., Molecular Genetics and Cell Biology, 1995

> Professional Experience:

• 2004-present Molecular Biologist, Principal Investigator, Biosciences Division, Argonne National Laboratory, Argonne, IL
• 2000 – 2004 Assistant Molecular Biologist, Biochip Technology Center, Argonne National Laboratory, Argonne, IL
• 1995 – 2000 Postdoctoral Fellow, Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, Argonne, IL
• 1982 – 1986 Student Researcher, Eleanor Roosevelt Institute for Cancer Research, Denver, CO

> Research Highlights:

I have a long-standing interest in delineating signal transduction pathways and isolating their associated molecular components in eukaryotic cells as they are induced to differentiate, either morphogenically or by maturation. Much of this research has focused on two systems: 1) signal transduction cascades involved in regulating the maturation of promyelocytic human leukemia cells into cells of distinct mature myelomonocytic phenotypes, and 2) understanding the molecular basis of branching morphogenesis and tubulogenesis of primary endothelial cells. I have taken a wide variety of molecular approaches to both identify and isolate mediators in these processes and to assign functional roles to proteins whose function has not been known. More recently, with the advent of high-resolution x-ray fluorescence imaging resources at the Advanced Photon Source, my research has become focused on understanding the role endogenous metals such as iron, copper, and zinc play in regulating protein activity and function during these cellular differentiation processes and in developing and utilizing methodologies for the characterization of the eukaryotic metalloproteome. In addition, we are currently developing cell biology tools to support the evolution of XRF imaging capabilities so as to maximize the utility of this unique research platform.

> Honors/Awards

• William H. Echols Scholar, University of Virginia
• Lucille P. Markey Fellow, University of Chicago
• Medical Advisory Board, Leukemia Research Foundation, 2001-present
• American Association for Cancer Research, 2002-present

> Grant Support

• Argonne LDRD WP-03315 “Functional metalloproteomics in angiogenesis” (D. Glesne, PI) 10/01/03-9/31/06
• The objective of this project is to identify and analyze the role specific metalloproteins play in regulating human angiogenesis.
• Argonne LDRD WP-03377 “Metalloregulation of Signalling (D.Glesne, PI) 10/01/04-9/31/07
  The objective of this project is to define the role metals play in regulating signal transduction cascades initiated by interaction between the extracellular matrix and human cell systems