PhD THESIS:
Raffen, R. 1993. Characterization of the recA protein metal-nucleotide binding site.
University of Illinois at Chicago, Chicago, IL.
PEER REVIEWED PUBLICATIONS:
1. Wilkins Stevens, P., Raffen, R., Hanson, D.K., Deng, Y. L., Berrios Hammond, M., Westholm, F.A., Murphy, C., Eulitz, M., Wetzel, R., Solomon, A., Schiffer, M., and Stevens, F.J. 1995. Recombinant immunoglobulin variable domains generated from synthetic genes provide a system for in vitro characterization of light chain amyloid proteins. Protein Sci. 4: 421-432.
2. Raffen, R., Wilkins Stevens, P., Schiffer, M., and Stevens, F.J. 1998. Reengineering immunoglobulin domain interactions by introduction of charged residues. Protein Eng. 11: 303-309.
3. Pokkuluri, P.R., Huang, D.B., Raffen, R., Cai, X., Johnson, G., Wilkins-Stevens, P., Stevens, F.J., and Schiffer, M. 1998. A domain flip as a result of a single amino-acid substitution. Structure. 6: 1067-1073.
4. Raffen, R., Dieckman, L.J., Szpunar, M., Wunschl, C., Pokkulur, P.R., Dave, P., Wilkins Stevens, P., Schiffer, M., and Stevens, F.J. 1999. Physicochemical consequences of amino acid variations that contribute to fibril formation by immunoglobulin light chains. Protein Sci. 8: 509-517.
5. Davis, D.P., Raffen, R., Dul, J.L., Vogen, S., Williamson, E.K., Stevens, F.J., and Argon, Y. 2000. Inhibition of amyloid fiber assembly by both BiP and its target peptide. Immunity 13: 433-442.
6. Kim Y.S., Cape S.P., Chi, E., Raffen R., Wilkins-Stevens P., Stevens F.J., Manning
M.C., Randolph T.W., Solomon A., Carpenter J.F. 2001. Counteracting effects of renal solutes on amyloid formation by immunoglobulin light chains. J. Biol. Chem. 276: 1626-1633.
7. Lin, Y-M., Raffen, R., Zhou, Y., Flavin, M.T., and Stevens, F.J. 2001. Amyloid fibril formation in microwell plates for screening of inhibitors. Amyloid 8: 182-193.
8. Davis, D.P., Gallo, G., Vogen, S.M., Dul, J.L., Sciarretta, K.L., Kumar, A., Raffen, R., Stevens, F.J., and Argon, Y. 2001. Both the environment and somatic mutations govern the aggregation pathway of pathogenic immunoglobulin light chain. J. Mol. Biol. 313: 1021-1034.
9. Pokkuluri, P.R., Gu, M., Cai, X., Raffen, R., Stevens, F.J., and Schiffer, M. 2002. Factors contributing to decreased protein stability when aspartic acid residues are in -sheet regions. Protein Sci. 11: 1687-1694.
10. Stols, L., Gu, M-Y., Dieckman, L., Raffen, R., Collart, F.R., and Donnelly, M.I. 2002. A new vector for high throughput, ligation independent cloning encoding a TEV protease cleavage site. Protein Expr. Purif. 25: 8-15
11. Pokkuluri, P.R., Raffen, R., Dieckman, L., Boogaard, C., Stevens, F.J., and Schiffer, M. 2002. Increasing protein stability by polar surface residues: Domain-wide consequences of interactions within a loop. Biophys. J. 82: 391-398.
INVITED BOOK CHAPTERS:
1. Raffen, R. and Stevens, F.J. 1999. Small-zone, high-speed gel filtration chromatography to detect protein aggregation associated with light chain pathologies. Meth. Enzymol. 309: 318-332.
2. Wilton, R. Myatt, E.A., and Stevens, F.J. 2003. Analysis of protein-protein interactions by simulation of small-zone gel filtration chromatography. in Methods in Molecular biology, vol. 261: Protein-Protein Interactions: Methods and Applications. (H. Fu, ed.) Humana Press, Totowa, NJ. (in press)
PATENTS:
1. Stevens, F.J., Raffen, R., Wilkins Stevens, P., and Schiffer, M. 2003. Method for altering antibody light chain interactions. No. 6,485,943
2. Stevens, F.J., Argon, Y., Davis, D., and Raffen, R. 2005. A fibril-blocking peptide, a method for preventing fibril formation. No. 6,878,521
INVENTION REPORTS:
1. Stevens, F.J. and Raffen, R.. 1998. In vitro protein fibril production: potential high-throughput method to screen for anti-amyloid drug leads. ANL-IN-98-046, DOE S91,201.
2. Stevens, F.J., Argon, Y., Davis, D., and Raffen, R. 1999. Composition of a fibril-blocking peptide; strategy for development of peptides to inhibit conformational disease resulting from protein domain swapping. ANL-IN-99-019, DOE S93,203.
3. Argon, Y., Davis, D.P., Stevens, F.J., and Raffen, R. 2000. A method for inhibiting amyloid-like protein aggregation with peptides. UCHI # 820.
CONTRIBUTED POSTERS AND TALKS
1. Raffen, R., Deng, Y.-L., Hanson, D.K., Myatt, E., Schiffer, M., Stevens, F.J., and Wilkins Stevens, P. 1993. Characterization of recombinant immunoglobulin VL domains expressed in E. coli. Amoco-sponsored University-Industry Poster Session, Naperville, IL, Oct 8.
2. Jiang, X.-L., Myatt, E.A., Raffen, R., and Stevens, F.J. 1995. Study of interactions between amyloid forming protein and glycosaminoglycans. Protein Interactions: Pfizer/Beckman Institute Protein Symposium, Urbana, IL, June 1-4.
3. Raffen, R., Wilkins Stevens, P., Hanson, D.K., Deng, Y., Berrios-Hammond, M., Westholm, F.A., Schiffer, M., and Stevens, F.J. 1996. In vitro characterization of light chain amyloidosis using recombinant light chain variable domains. Biophysical J. 70 (2): SU190-SU190 Part 2. Biophysical Society 40th Annual Meeting, Baltimore, MD, Feb 17-21.
4. Schiffer, M., Huang, D.-B., Raffen, R., Pokkuluri, R., Wilkins Stevens, P., and Stevens, F.J. 1997. Major unexpected effect of a Gln to Glu substitution on a dimer interface. 1997 Proteins Gordon Conference.
5. Raffen, R., Dieckman, L.J., Wilkins Stevens, P., Pokkuluri, P.R., Schiffer, M., and Stevens F.J. 1997. Characterizing primary structural determinants of immunoglobulin light chain amyloidosis with recombinant light chain variable domains. FASEB Summer Research Conference: Amyloid and other Abnormal Protein Assembly, Copper Mt., CO, July 13-18.
6. Pokkuluri, P.R., Huang, D.-B., Raffen, R., Wilkins Stevens, P., Stevens, F.J., and Schiffer, M. 1997. How do metal ions help promote the formation of protein crystals? Annual American Crystallographic Association Meeting, St. Louis, MO, July 19-25.
7. Schiffer, M., Pokkuluri, P.R., Cai, X., Johnson, G., Raffen, R., and Stevens, F.J. 1998. A single amino acid substitution changes the mode of dimerization. Third Annual Structural Biology Symposium, University of Texas Medical Branch at Galveston, April 3-5.
8. Raffen, R., Pokkuluri, P.R., Wilkins Stevens, P., Schiffer, M., and Stevens, F.J. 1998. Characterization of immunoglobulin light chain amyloidosis by site-directed mutagenesis. 12th Symposium of the Protein Society, San Diego, CA, July 25-29.
9. Stevens, F.J., Raffen, R., Dieckman, L.J., Szpunar, M., Pokkuluri, P.R., Wilkins Stevens, P., and Schiffer,M. 1999. Characterization of immunoglobulin light chain pathogenic aggregation and amyloidosis by site-directed mutagenesis. 4th Annual Midwest Stress Response and Chaperone Meeting, Jan 16.
10. Davis, D.P., Vogen, S., Case, C., Raffen, R., Stevens, F., and Argon, Y. 1999. Unlocking the mechanisms of immunoglobulin aggregation disorders. 4th Annual Midwest Stress Response and Chaperone Meeting, Jan 16.
11. Schiffer, M., Pokkulur, P.R., Raffen, R., Cai, X., Johnson, G., and Stevens, F.J. 1999. Alteration of dimerization mode by single amino substitutions. 13th Symposium of the Protein Society, Boston, MA, July 24-28.
12. Dong, A., Kim, Y.-S., Raffen, R., Randolph, T.W., Solomon, A., Wilkins Stevens, P., Stevens, F.J., and Carpenter, J.F. 2000. Entrapping aggregation intermediates of -helix and -domain proteins by thermochemical denaturation: an infrared spectroscopic study. FASEB Conference: Amyloid and other Abnormal Protein Assembly Processes, Copper Mountain, CO.
13. Kim, Y.-S., Cape, S.P., Chi, E., Raffen, R., Wilkins Stevens, P., Stevens, F.J., Solomon, A., Manning, M.C., Randolph, T.W., and Carpenter, J.F. 2000. Counteracting effects of renal solutes on amyloid fibril formation by immunoglobulin light chains. FASEB Conference: Amyloid and other Abnormal Protein Assembly Processes, Copper Mountain, CO.
14. Wan, K., Duan, M., Lin, Y.-M.; Lin, J., Raffen, R., and Stevens, F.J. 2001. The Study of Protein-Protein Interactions by H/D Exchange and ESI MS. 49th Annual Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 27-31.
15. Raffen, R. 2002. Protein Conformation and Disease. LDRD Mini Symposium, May 14.
16. Wilton, R. , Saxena, P., and Stevens, F.J. 2004. Expression, purification, and biochemical characterization of human receptor for advanced glycation endproducts (RAGE) from Escherichia coli. Protein Sci 13: Suppl. 1:329. 18th Symposium of the Protein Society, San Diego, CA, August 14-18.
