The vectors
pMCSG7 and pMCSG19 are used at Argonne
National Laboratory for the routine, high-throughput
production of single proteins. Soluble
proteins are produced from pMCSG7, which
appends a his-tag followed by a TEV protease
cleavage site, and poorly soluble proteins
are made from pMCSG19. In the presence
of TVMV protease (from pRK1037, see
David Waugh's web
site), pMCSG19 generates untagged
MBP and his-tagged target protein. The
vector pMCSG9 generates proteins appended
with his-tagged MBP followed by a TEV protease
site. Both the MBP vectors can improve
the solubility of some proteins. |
pMCSG7
pMCSG19
pMCSG9
To download GB files, right click (ctrl click Mac). Open with sequence editor or text editor.
References
pMCSG7: Stols et al. (2002) Protein Expr. Purif. 25, 8-15 Medline
pMCSG19: Donnelly et al. (2006) Protein Expr. Purif. In Press
pMCSG9: Donnelly et al. (2006) Protein Expr. Purif. In Press
in vivo proteolysis with pMCSG19 requires pRK1037 (David
Waugh laboratory)
Nallemsety et al. (2005) Protein Expr. Purif.38,
108-115 Medline
